Characterization of phosphoenolpyruvate synthase mutants inSalmonella typhimurium
โ Scribed by J. Robert Smyer; Randall M. Jeter
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 722 KB
- Volume
- 153
- Category
- Article
- ISSN
- 0302-8933
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๐ SIMILAR VOLUMES
A mutant of Saccharomyces cerevisiae lacking phosphoenolpyruvate carboxykinase (E.C. 4.1.1.32) was isolated. The mutant did not grow on gluconeogenic sources except glycerol. The mutation was recessive and apparently affected the structural gene of the enzyme. Intracellular levels of metabolites rel
The expression of an acetolactate synthase (ALS) gene isolated from the cruciferous plant Brassica napus was investigated in Salmonella typhimurium. Using an expression plasmid containing the highly active trc (trp-lac) promoter, several plant ALS constructs were made containing successive in-frame