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Isolation and characterization of a mutant ofSaccharomyces cerevisiaedefective in phosphoenolpyruvate carboxykinase

✍ Scribed by Javier Perea; Carlos Gancedo

Publisher
Springer
Year
1982
Tongue
English
Weight
283 KB
Volume
132
Category
Article
ISSN
0302-8933

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✦ Synopsis

A mutant of Saccharomyces cerevisiae lacking phosphoenolpyruvate carboxykinase (E.C. 4.1.1.32) was isolated. The mutant did not grow on gluconeogenic sources except glycerol. The mutation was recessive and apparently affected the structural gene of the enzyme. Intracellular levels of metabolites related to the metabolic situation of the enzyme were not significantly affected after transfer of the mutant from a medium with glycerol to a medium with ethanol as carbon source. In these conditions only AMP decreased 3 to 5 times. A search for mutants affected in the other gluconeogenic enzyme, fructose 1,6 bisphosphatase, remained unsuccessful.

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