✦ LIBER ✦

Characterization of monoclonal antibodies against human rotavirus hemagglutinin

✍ Scribed by Setsuko Kitaoka; Norio Fukuhara; Fumiyo Tazawa; Hiroshi Suzuki; Tetsuo Sato; Dr. Tasuke Konno; Takusaburo Ebina; Nakao Ishida

Publisher: John Wiley and Sons
Year: 1986
Tongue: English
Weight: 754 KB
Volume: 19
Category: Article
ISSN: 0146-6615
DOI: 10.1002/jmv.1890190404

No coin nor oath required. For personal study only.

✦ Synopsis

Three monoclonal antibodies capable of specifically inhibiting hemagglutination of human rotavirus were produced. Their hemagglutination inhibition (HI) activity was specific to the homologous strain (KUN) used for immunization. The monoclonal antibodies with HI activity were highly effective in neutralizing the infectivity of the KUN strain. These antibodies reacted with Vp80, and 80,000 molecular weight (MW) protein present in the viral outer shell. It was confirmed by immunoblotting assay with the monoclonal antibodies that the antigenic site of human rotavirus hemagglutinin (HA) resides on Vp80 and on its smaller trypsin cleavage products Vp30 (MW 30,000) and Vp24 (MW 24,000). Immunofluorescence studies using the antibodies revealed that the HA antigen of the KUN strain developed at the final stage of virus maturation.

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