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Characterization of methacrylate monoliths for purification of DNA molecules

✍ Scribed by Mojca Benčina; Aleš Podgornik; Aleš Štrancar

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
717 KB
Volume
27
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

The suitability of methacrylate based anion exchange monolithic supports for the separation and purification of plasmid and genomic DNA has been explored. The effect of the size of the channels, ionic strength of the solution, and ligand density on the dynamic binding capacity has been investigated. The dynamic binding capacity was found to be flow independent, at least up to a linear velocity of 700 cm h^–1^, and exceeded 9 mg mL^–1^ for all types of DNA. The recovery depends on the pH value of the mobile phase and its ionic strength as well as on the density of the active groups. Under optimal conditions recoveries exceeding 80% were obtained even for genomic DNA. Finally, the suitability of this approach is demonstrated by purification of a real‐life sample.

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