Characterization of lidocaine and its metabolites in human plasma using capillary electrophoresis

✍ Scribed by Magnus S. Anderson; Hong Wan; Mohamed Abdel-Rehim; Lars G. Blomberg

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 138 KB
• Volume: 11
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/(sici)1520-667x(1999)11:8<620::aid-mcs8>3.0.co;2-8

✦ Synopsis

A method for the separation of lidocaine and six of its metabolites in plasma has been developed. Separation was performed using a buffer consisting of Ž . 35 mM phosphaterTris pH 3.0, 6 mM cetyltrimetylammoniumbromid CTAB and Ž . 9% vrv methanol. The key variables, CTAB and methanol concentrations, were optimized by the application of a factorial design. Rinsing the capillaries with ethanol between runs resulted in highly reproducible migration times, relative standard deviation 0.69%. Under the conditions applied, the seven analytes could be separated with high efficiency and resolution within 8.1 min. Overall performance, as demonstrated in validation experiments, was excellent.