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Characterization of human sperm plasma membrane: Glycolipids and polypeptides

✍ Scribed by Mack, S. R. ;Zaneveld, L. J. D. ;Peterson, R. N. ;Hunt, W. ;Russell, L. D.

Publisher: John Wiley and Sons
Year: 1987
Tongue: English
Weight: 667 KB
Volume: 243
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1402430219

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✦ Synopsis

The plasma membranes from ejaculated human spermatozoa were removed by nitrogen cavitation (600 PSI for 10 min) and isolated by centrifugation followed by a discontinuous sucrose density gradient centrifugation. Glycolipid analysis of the plasma membrane revealed a three-fold enrichment in gangliosides: GM3 and GD1a/GD1b and neutral glycolipids: globoside and sulfatide as compared to that of whole human sperm. Two dimensional electrophoresis of human sperm plasma membranes revealed about 75 polypeptides. Several of these polypeptides were similar in migration and in display of shape and color to that found in boar sperm plasma membranes.

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