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Characterization of granulocytes and mast cells in cultures of mouse bone marrow stimulated with interleukin-3

✍ Scribed by James G. Bender; Dennis E. Van Epps; Carleton C. Stewart

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
780 KB
Volume
135
Category
Article
ISSN
0021-9541

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✦ Synopsis

Bone marrow cells in liquid culture with interleukin 3 produce a population of non-adherent granulocytes and mast cells. Flow cytometry was used to identify granulocytes and mast cells on the basis of the physical properties of perpendicular light scatter (PLS) and coulter volume (CV) as well as the expression IgE and CR3 receptors. Muhicolor analysis indicated there were subpopulations of Thyl.2 positive cells which transiently appeared in these cultures and also expressed IgE receptors, CR3 receptors or neither of these receptors. The data suggested a differentiation scheme in which Thyl.2 positive precursor cells give rise to granulocytes and mast cells. Further evidence for this differentiation scheme was provided from CV vs. PLS distributions which showed increases in CV and PLS as Thyl.2 positive cells differentiated into mast cells and decreases in CV and PLS as Thyl.2 positive cells differentiated into granulocytes.

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