Characterization of electrogenerated peroxyoxalate chemiluminescence

✍ Scribed by Katrina F. Heinze; Timothy A. Nieman

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 544 KB
• Volume: 284
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)85318-e

✦ Synopsis

Peroxyoxalate chemiluminescence (PO-CL) is a commonly used chemiluminescent detection method for liquid chromatography . This paper concerns the electrogeneration of peroxyoxalate chemiluminescence (PO-ECL) as a means to eliminate post-column reagent addition which is required with conventional peroxyoxalate chemiluminescence detection in liquid chromatography . In acetonitrile, peroxyoxalate chemiluminescence can be electrogenerated at potentials between -1 .5 and -1 .9 V (versus Ag/ AgC1) . Imidazole, a catalyst for PO-CL, was found to interfere with the electrochemistry of the model peroxyoxalate and thus the emission of light with PO-ECL . Conversely, PO-ECL is enhanced in the presence of hydrogen peroxide . This enhancement is time dependent . Direct comparison showed PO-ECL to be less sensitive than PO-CL .