Characterization of DNA topoisomerase I from Mycobacterium tuberculosis: DNA cleavage and religation properties and inhibition of its activity
β Scribed by Godbole, Adwait Anand; Leelaram, Majety Naga; Bhat, Anuradha Gopal; Jain, Paras; Nagaraja, Valakunja
- Book ID
- 119181406
- Publisher
- Elsevier Science
- Year
- 2012
- Tongue
- English
- Weight
- 734 KB
- Volume
- 528
- Category
- Article
- ISSN
- 0003-9861
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We have purified a topoisomerase activity from broccoli (Brassica oleracea var. italica) to near homogeneity. The enzyme is an 80 kDa monomer as judged by gel filtration chromatography and SDS gel electrophoresis, though it may represent a proteolytic fragment of a larger protein. The enzyme is capa
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