Characterization of Cytokinins by Electrospray Ionization and Collision-induced Dissociation Mass Spectrometry Without Precursor Ion Selection

✍ Scribed by Bartók, T.; Börcsök, G.; Komoróczy, R.; Sági, F.

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 455 KB
• Volume: 31
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/(sici)1096-9888(199607)31:7<756::aid-jms348>3.0.co;2-l

✦ Synopsis

Eight naturally occurring and four synthetic cytokinins were studied by means of electrospray ionization collisioninduced dissociation single-quadruple mass spectrometry in order to examine the potential of this technique in the mapping of their structures. At a low capillary exit voltage, protonated molecular ions dominated, but in the range 240-300 V informative and typical collision-induced fragmentation was obtained. At these voltages, protonated adenine and purine ions (mlz 136 and 119, respectively) were formed from all natural but not from the synthetic cytokinins, which produced other ions specific to the molecule. Nevertheless, the loss of 132 u ([M + H -C,H,O,I +) or 162 u (I M + H -C,H,,O,] +) pointed to the presence of the ribosyl or glucosyl group in the two types of cytokinins. The occurrence of the molecular and 'diagnostic' ions, and the possible loss of 132 or 162 u, readily permit the characterization of the chemical structure and the identification of unknown cytokinin molecules.