Characterization of chloride uptake in Drosophila Kc cells
โ Scribed by Ann C. Sherwood; Kathleen John-Alder; Marilyn M. Sanders
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 771 KB
- Volume
- 136
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
โฆ Synopsis
Drosophila Kc cells use at least two mechanisms for chloride uptake. These transport systems can be distinguished by their kinetic properties and by their differential sensitivity to various drugs. One transport system predominates at [CI-1, below 30 mM and is greater than fivefold more sensitive to disulfonic stilbenes than the second system. At [CI-1, above 30 mM, the predominant uptake mechanism is inhibited by vanadate and nitrate.
To whom reprint requests/correspondence should be addressed.
๐ SIMILAR VOLUMES
K,-cells from Drosophila melanogaster, grown under serum-free conditions, produce two p-hexosaminidases and secrete these enzymes into the medium. The two enzymes were separated by DEAE-exchange chromatography. According to their substrate specificities one enzyme is a f3-N-acetyl-D-glucosarninidase
Kc-cells from Drosophila produce two different beta-D-hexosaminidases, a beta-N-acetyl-D-glucosaminidase (E.C.3.2.1.30) and a beta-N-acetyl-D-hexosaminidase (E.C.3.2.1.52), which are also secreted into the medium. The Mr of both enzymes is about 126,000 +/- 9,700; the S-values are 8.37 +/- 0.44. Bot