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Characterization of callus formation and camptothecin production by cell lines ofCamptotheca acuminata

✍ Scribed by Arjon J. Hengel; Marien P. Harkes; Harry J. Wichers; Paul G. M. Hesselink; Reinetta M. Buitelaar

Publisher
Springer
Year
1992
Tongue
English
Weight
528 KB
Volume
28
Category
Article
ISSN
0167-6857

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✦ Synopsis

Camptotheca acuminata plants contain camptothecin which is a secondary metabolite with strong anti-tumor activity. The induction of callus and cell suspension cultures from Camptotheca acuminata stem parts on different media is described. Growth in the original media and in media with different salts and/or vitamins was followed by measuring several growth parameters. Camptothecin was detected and identified by means of TLC, HPLC and GC-MS. The production of camptothecin in cell suspension cultures was followed and compared to the original plant material. Results indicate that high density cultures could be easily established, producing ca I mg 1-1 of camptothecin.

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