The nar promoter of Escherichia coli, which is maximally induced under anaerobic conditions in the presence of nitrate, was characterized to see whether the nar promoter cloned onto pBR322 can be used as an inducible promoter. To increase the expression level, the nar promoter was expressed in โฌ. co
Characterization of an oxygen-dependent inducible promoter, the Escherichia coli nar promoter, in gram-negative host strains
โ Scribed by Kil Ho Lee; Moo Hwan Cho; Taeowan Chung; Ho Nam Chang; Sun Ha Lim; Jongwon Lee
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 146 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0006-3592
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A nar promoter system (a modified nar promoter in a mutant host Escherichia coli (pMW618/W3110narL(-))), which is maximally induced under microaerobic conditions, was developed and characterized through batch and fed-batch culture to see whether the modified nar promoter can be used as an oxygen-dep
The nar promoter of Escherichia coli is maximally induced under anaerobic or microaerobic conditions in the presence of nitrate. We previously demonstrated in batch experiments that the intact nar promoter of E. coli cloned into a pBR322-based plasmid serves as a high-level expression system in a na