A nar promoter system (a modified nar promoter in a mutant host Escherichia coli (pMW618/W3110narL(-))), which is maximally induced under microaerobic conditions, was developed and characterized through batch and fed-batch culture to see whether the modified nar promoter can be used as an oxygen-dep
โฆ LIBER โฆ
Characterization of an oxygen-dependent inducible promoter, the nar promoter of Escherichia coli, to utilize in metabolic engineering
โ Scribed by Se Jong Han; Ho Nam Chang; Jongwon Lee
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 133 KB
- Volume
- 72
- Category
- Article
- ISSN
- 0006-3592
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The nar promoter of Escherichia coli is maximally induced under anaerobic or microaerobic conditions in the presence of nitrate. We previously demonstrated in batch experiments that the intact nar promoter of E. coli cloned into a pBR322-based plasmid serves as a high-level expression system in a na