Characterization of an embryonal rhabdomyosarcoma cell line showing amplification and over-expression of the N-myc oncogene

✍ Scribed by Yasuhide Hayashi; Tohru Sugimoto; Yoshihiro Horii; Hajime Hosoi; Johji Inazawa; John T. Kemshead; Toshiya Inaba; Ryoji Hanada; Keiko Yamamoto; Allen M. Gown; Tadashi Sawada

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: French
• Weight: 815 KB
• Volume: 45
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910450423

✦ Synopsis

A parent rhabdomyosarcoma cell line designated SCMC-RM2 was established from bone-marrow tumor cells taken from an I I-year-old girl with an embryonal rhabdomyosarcoma. Subsequently a cloned SCMC-RM2-I cell line was isolated from a parent line. These cell lines grew as adherent monolayers in liquid culture with a doubling time of 50 and 52 hr, respectively. In addition, colonies were established in soft agar, which grew in a dose-dependent fashion with a cloning efficiency of 0.7 and 0.8%, respectively. Chromosomal analysis showed these cell lines had neither double minutes nor homogeneously staining regions. Chromosome number ranged from 61 to 93, translocation; t(9;13Xp22;q 14) was identified, and no alteration of chromosome 2 was observed. Surface membrane antigen profile of parent and cloned lines by using a panel of 24 monoclonal antibodies (MAbs) excluded the possibility of these being neuroblastoma cell lines. In addition, MAbs to the cytoplasmic protein desmin, myoglobin, muscle actin (a and y) and a-sarcomeric actin reacted with these cell lines, SCMC-RM2 and SCMC-RM2-I being thus identified as rhabdomyosarcorna. Southern blot analyses revealed 8and 7-fold amplification of the N-myc gene in SCMC-RM2 and SCMC-RM2-I as compared with the promyelocytic cell line HMO. Over-expression of the N-rnyc mRNA was noted over control cell lines.