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Characterization of a Tn925-induced mutant of Staphylococcus aureus altered in exoprotein production

✍ Scribed by Ana T. Giraudo; Gabriela L. Martínez; Aldo Calzolari; Dr. Rosa Nagel

Book ID
102910422
Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
533 KB
Volume
34
Category
Article
ISSN
0233-111X

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✦ Synopsis

An insertional mutant was isolated from a bovine Staphylococcus aureus strain after membrane-mating with a Streptacoccus faecalis strain carrying conjugative plasmid pCFl0 : : Tn925. This mutant, designated RC 128, showed enhanced production of cc-hemolysin and proteases and decreased production of coagulase, extracellular protein A, DNase, lipase and &hemolysin. N o difference was found in the production of p-hemolysin. Both, Southern blot analysis and transfer of the pleiotropic mutant phenotype by transduction, indicated that the mutation was originated from a single insertion of transposon Tn925. The LD.50 determined by intraperitoneal administration in mice showed that mutant RC128 was slightly less virulent than its parental strain.

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