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Characterization of a Temperature-Sensitive Mutant of Mouse FM3A Cells Defective in DNA Replication

โœ Scribed by Yasufumi Murakami, Hideyo Yasuda, Hiroshi Miyazawa, Fumio Hanaoka and Masa-Atsu Yamada


Book ID
123631830
Publisher
National Academy of Sciences
Year
1985
Tongue
English
Weight
863 KB
Volume
82
Category
Article
ISSN
0027-8424

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DNA replication and transcription in a t
โœ Mirkin, S. M. ;Shmerling, Zh. G. ๐Ÿ“‚ Article ๐Ÿ“… 1982 ๐Ÿ› Springer ๐ŸŒ English โš– 532 KB

A temperature-sensitive mutant of E. coli with a defective DNA gyrase B subunit has been obtained. The mutation is expressed in the thermolability of DNA gyrase in vitro and in DNA relaxation in vivo. DNA replication in the mutant does not stop under non-permissive conditions; its rate gradually fal