Characterization of a plasmid replicative origin from an extreme thermophile
✍ Scribed by Myriam de Grado; Iñigo Lasa; José Berenguer
- Book ID
- 109322376
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 484 KB
- Volume
- 165
- Category
- Article
- ISSN
- 0378-1097
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
## Abstract Caldolysin, the extracellular thermostable metal‐chelator‐sensitive lytic protease from __Thermus__ T‐351 was immobilized to Sepharose 4B, CM‐cellulose, and controlled pore glass (CPG). Although protein binding efficiencies were high (96, 88, and 95%), some loss of enzyme activity occur
We constructed a series of defective mini-F plasmids, which have deletion(s) in the replication origin I and/or origin II, and their derivatives, which do not produce F3 protein, by insertion of the XhoI fragment of Tn5 into the XhoI site at 41.0 F (kilobases on the coordinate map of F-plasmid). Usi