Characterization of a Panel of Somatic Cell Hybrids for Regional Mapping of the Mouse X Chromosome
β Scribed by Philip Avner, Danielle Arnaud, Laurence Amar, Jacques Cambrou, Heinz Winking and Liane B. Russell
- Book ID
- 123634529
- Publisher
- National Academy of Sciences
- Year
- 1987
- Tongue
- English
- Weight
- 973 KB
- Volume
- 84
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/30031
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We have characterized 19 DNA fragments originating from the human X chromosome. Most of them have been isolated from an X chromosome genomic library (Davies et al. 1981) using a systematic screening procedure. These DNA probes have been used to search for restriction fragment length polymorphisms (R
Mouse/hamster somatic cell hybrids form a valuable resource for mouse gene mapping. Characterization of these hybrids by isozyme analysis can be technically demanding and time-consuming. Speciesspecific polymerase chain reaction (PCR), where a mouse gene but not its homolog in the hamster is amplifi