✦ LIBER ✦

Characterization of a nucleic acid probe for the diagnosis of human coronavirus 229E infections

✍ Scribed by S. Myint; D. Harmsen; T. Raabe; S. G. Siddell

Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 910 KB
Volume: 31
Category: Article
ISSN: 0146-6615
DOI: 10.1002/jmv.1890310216

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A cDNA copy of the HCV229E nucleocapsid protein gene was isolated and characterized. Sequence analysis predicts a nucleocapsid polypeptide of 389 amino acids with a molecular weight (mol. wt.) of 43,450. Single strand RNA probes derived from the cDNA copy hybridize specifically to HCV229E RNA and approximately 50 pg of intracellular viral RNA can be readily detected. The application of nucleic acid hybridization as a routine procedure for the diagnosis of HCV229E infection is discussed.

📜 SIMILAR VOLUMES

Non-radioactive nucleic acid probes for

Non-radioactive nucleic acid probes for the diagnosis of virus infections

✍ H. G. Pereira 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 462 KB 👁 1 views

Nucleic acid hybridization is being increasingly used in viral diagnosis. Most of the assays described so far for this purpose require the use of radioactive probes. Their replacement by \*adioactive assays has many advantages and makes the technique feasible in routine diagnostic work. Non-radioact

Evaluation of antibody-based and nucleic

Evaluation of antibody-based and nucleic acid-based assays for diagnosis of hepatitis E virus infection in a rhesus monkey model

✍ Jun Zhang; Sheng X. Ge; Guo Y. Huang; Shao W. Li; Zhi Q. He; Ying B. Wang; Ying 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 123 KB 👁 1 views

## Abstract We have evaluated four hepatitis E virus (HEV) specific antibody assays, using sequential samples taken from 86 rhesus monkeys at intervals for up to 86 weeks after they had been infected with different doses of HEV. The animals are a common experimental model of hepatitis E. The large

A Single Molecular Beacon Probe Is Suffi

A Single Molecular Beacon Probe Is Sufficient for the Analysis of Multiple Nucleic Acid Sequences

✍ Yulia V. Gerasimova; Aaron Hayson; Jack Ballantyne ; Dmitry M. Kolpashchikov 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 504 KB

Performance of a commercial nucleic acid

Performance of a commercial nucleic acid amplification test with extrapulmonary specimens for the diagnosis of tuberculosis

✍ C. Piersimoni; S. Bornigia; G. Gherardi 📂 Article 📅 2011 🏛 Springer 🌐 English ⚖ 130 KB

A Sandwiched Biological Fluorescent Prob

A Sandwiched Biological Fluorescent Probe for the Diagnosis of Human Ovarian Tumor Based on TiO2Nanoparticles

✍ Peisi Zhu; Shasheng Huang; Mengyao Li; Na Ding; Bing Peng; Lingmi Kong; Yang Bo 📂 Article 📅 2010 🏛 Springer 🌐 English ⚖ 357 KB

Efficacy of a recombinant Norwalk virus

Efficacy of a recombinant Norwalk virus protein enzyme immunoassay for the diagnosis of infections with Norwalk virus and other human “candidate” caliciviruses

✍ Simon Parker; David Cubitt; Jason Xi Jiang; Mary Estes 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 536 KB

## Abstract A recombinant Norwalk virus antigen enzyme immunoassay (rNV EIA) for the detection of Norwalk virus (NV) antibodies was evaluated using sera from volunteers and patients infected with Norwalk, Hawaii, Snow Mountain Agent, small round structured viruses (SRSV), and human calicivirus (HuC