Characterization of a hepatic proliferation inhibitor (HPI): Effect of HPI on the growth of normal liver cells—comparison with transforming growth factor beta

✍ Scribed by Anthony C. Huggett; Henry C. Krutzsch; Snorri S. Thorgeirsson

• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: English
• Weight: 598 KB
• Volume: 35
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.240350405

✦ Synopsis

Improvements in the purification of a hepatic proliferation inhibitor (HPI) from adult rat liver have yielded a product that has an inhibitory activity 1,000-fold greater than previously reported. The growth inhibitory activity, which could be eluted from SDS-PAGE at 17-19 kilodaltons (kD), was compared to that of transforming growth factor beta (TGF-P). The IDso of the HPI preparation in Fischer rat liver epithelial cells was 50 pg/ml (2.5 pM) compared to a value of 260 pg/ml (10.4 pM) obtained for pure human TGF-P. Both inhibitors also modulated the -stimulation of DNA synthesis in primary hepatocytes by either epidermal growth factor or a growth stimulatory activity prepared from serum of hepatectomized rats. The ID5@ of HPI and TGF-/3 in these cells were 250 p g / d and 40 pglml, respectively. In contrast to TGF-/3 the growth inhibitory activity of HPI was unaltered in the presence of an antibody raised against TGF-8. The possible mechanism of action of HPI is discussed.