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EFFECT OF THE INTERACTION BETWEEN TRANSFORMING GROWTH FACTOR β AND ERYTHROPOIETIN ON THE PROLIFERATION OF NORMAL ERYTHROID PROGENITORS AND LEUKEMIC UT-7 CELLS: ACTION OF TRANSFORMING GROWTH FACTOR β ON THE ERYTHROPOIETIN RECEPTOR

✍ Scribed by E. LEVEQUE; M. D. NAGEL; B. HAYE

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
679 KB
Volume
14
Category
Article
ISSN
0278-0232

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✦ Synopsis

The actions of transforming growth factor , 8 (TGFP) and erythropoietin (Epo) were studied using normal erythroid progenitors from fetal rat liver and spleen at 18, 19 and 20 days. rhTGFbl inhibited the growth of late BFUe colonies significantly at each age and in both organs in methylcellulose cultures containing 2 U/ml rhEpo. There was no significant inhibition of CFUe proliferation, except for spleen CFUe at 18 days, suggesting different CFUe sensitivities to growth factors at a given fetal age, 18 days, in liver and spleen. The colorimetric MTT assay was used to examine the inhibition of the growth of human leukemic UT-7 cells by TGFP1. TGFPl inhibited the proliferation of UT-7 cells in cultures without Epo at 24 h and in cultures with Epo at 24 and 72 h. The specific binding of [1251]Epo to UT-7 surface was decreased by TGFPl without any change in non-specific binding. TGFPl also inhibited the expression of Epo-receptors on UT-7 cells, without changing receptor affinity. The inhibition of hematopoietic progenitor cell growth by TGFP could involve altering the cell surface expression of growth factor receptors.

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