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Characterization of a Gene Encoding a Pichia pastoris Protein Disulfide Isomerase

✍ Scribed by Abdirashid Warsame; Randi Vad; Tom Kristensen; Tordis Beate Øyen

Book ID
115587561
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
302 KB
Volume
281
Category
Article
ISSN
0006-291X

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We purified a 58 kDa serine protease from culture-supernatant of Pichia pastoris and found that the NH,-terminal amino acid sequence of this protease is closely homologous to that of mature protein of Saccharomyces cerevisiae carboxypeptidase Y (CPY), which is encoded by the PRCl gene. Using the S.