Characterization of a chloroplast DNA sequence fromChlorella ellipsoideathat promotes autonomous replication in yeast

✍ Scribed by Takashi Yamada; Miyuki Shimaji; Yuji Fukuda

• Book ID: 104613898
• Publisher: Springer
• Year: 1986
• Tongue: English
• Weight: 999 KB
• Volume: 6
• Category: Article
• ISSN: 0167-4412
• DOI: 10.1007/bf00015230

✦ Synopsis

An EcoRI 2.7 kbp fragment from Chlorella ellipsoidea chloroplast DNA (cpDNA) cloned in YIp5 was shown to promote autonomous replication in Saccharomyces cerevisiae. The fragment was localized in the small single copy region close to the inverted repeat. The ARS activity (autonomously replicating sequences in yeast) was found to be confined within a subclone of a ca. 300 bp HindIlI fragment. Sequence analysis of this fragment revealed its high AT content and the presence of several direct and inverted repeats and a few elements that were related to the yeast ARS consensus sequence. Electron microscopic studies revealed that this sequence did not coincide with the primary replication origin of chloroplast DNA. The functioning of this sequence as a possible origin of plasmid replication in vivo is discussed. This is the first report on Chlorella cpDNA sequence.