Nucleotide sequences and stability of aNicotiananuclear DNA segment possessing autonomously replicating ability in yeast

✍ Scribed by Takeshi Ohtani; Shigeot Kiyokawa; Toshifumi Ohgawara; Hiroshi Harada; Hirofumi Uchimiya

• Publisher: Springer
• Year: 1985
• Tongue: English
• Weight: 285 KB
• Volume: 5
• Category: Article
• ISSN: 0167-4412
• DOI: 10.1007/bf00017871

✦ Synopsis

The nucleotide sequence of a tobacco (Nicotiana tabacum) chromosomal DNA segment (t3-ars) capable of replication in yeast (ars: autonomously replicating sequences) is presented. The subcloned region (618 bp) contained 11 bp consensus (5' A/TTTTATPuTTTA/T 3') essential for several yeast ars, and 73% A and T. Unique 70 bp repetitive sequences resided next to this sequence. Thirty-two bp AT repeats were also seen in the neighbourhood of the repetitive sequence. The hybrid plasmid containing t3-ars was mitotically stabilized by the help of yeast centromere (CEN4).