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Characterization of 3ll-tumor variants generated by in vitro macrophage-mediated selection

✍ Scribed by Linda M. Remels; Patrick C. de Baetselier

Publisher
John Wiley and Sons
Year
1987
Tongue
French
Weight
985 KB
Volume
39
Category
Article
ISSN
0020-7136

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✦ Synopsis

Following sequential interactions between activated syngeneic Mas and 3LL tumor cells, stable M*-resistant 3LL variants were isolated. Unlike the unselected 3LL cells, these Maselected variants were relatively resistant to the cytostatic and cytolytic activity of activated effector Mas. Such Maresistant 3LL variants evade the Ma tumoricidal activity by at least two mechanisms. Firstly, they manifest a reduced susceptibility towards Ma-related cytotoxins such as TNF. Secondly, they actively suppress the cytotoxic potential of Mas through secretion of Ma-inhibitory factors. The resistance of the 3LL variants to M+ effector cells in vitro was reflected in vivo by a higher tumorigenic and metastatic potential. No strict correlation was found between the NK sensitivity of MCresistant and MCsensitive 3LL cells and their metastatic ability. Hence, activated tumoricidal Mas may play a central role in either the elimination or selection of neoplastic cells. 'To whom reprint requests should be sent, at the Instituut voor Moleculaire Biologie,

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