Characterization of 18 new BoLA-DRB3 alleles
β Scribed by J-C. Maillard; C. Renard; P. Chardon; 1I. Chantal; A. Bensaid
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 227 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0268-9146
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The products of the __BoLAβDRB3__ locus are important molecules in the bovine immune response. Several techniques have been used to study and define this locus but they are generally time consuming and limited in their ability to detect novel alleles. In this study we used denaturing gradient gel el
## Summary Bovine MHC (__BoLA__β) __DRB3__ alleles encoded by the __DH8A__, __DH22A__ and __DH24A__ class II haplotypes were cloned from cDNA and characterized by sequence analysis. Comparison with other fullβlength __DRB3__ sequences suggested that __DRB3__ alleles may have evolved through multipl
Potential associations were investigated between bovine leucocyte antigen (BoLA) alleles and occurrence of disease. Cows (Holstein n = 835; Jersey n = 66) were examined for polymorphisms of the second exon of the BoLA-DRB3 gene, using the polymerase chain reaction (PCR), followed by digestion of the