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Characterization by gas-liquid chromatography-mass spectrometry of oligosaccharides resulting from the hydrazinolysis-nitrous acid deamination reaction of glycopeptides

✍ Scribed by Gérard Strecker; Annick Pierce-Cretel; Bernard Fournet; Geneviève Spik; Jean Montreuil

Publisher: Elsevier Science
Year: 1981
Tongue: English
Weight: 532 KB
Volume: 111
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(81)90222-0

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✦ Synopsis

The hydrazinolysis-nitrous acid deamination of glycopeptides leads to the specific cleavage of N-acetylglucosamine linkages. The 2,5-anhydro-mannose containing oligosaccharides obtained by this way are reduced by sodium borohydride, methylated, and analyzed by gas-liquid chromatography-mass spectrometry. Here we report the characterization of 11 oligosaccharides obtained from various fucosyl-glycoasparagines and glycopeptides. In addition, the structure of a partially characterized glycopeptide isolated from human milk sIgA was investigated as an application of the present method.

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