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Characterization and purification of thermostable β-glucosidase from Clostridium thermocellum

✍ Scribed by N. Aït; N. Creuzet; J. Cattanéo


Book ID
118313624
Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
685 KB
Volume
90
Category
Article
ISSN
0006-291X

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Acetate kinase (EC 2.7.2.1), an enzyme involved in the wasteful production of acetate during conversion of cellulose to ethanol by Clostridlum thermocellum, was purified 144-fold. The enzyme has an Mr of 84 kD by non-denaturing gradient gel electrophoresis, and an Mr of 46 kD when estimated with a d