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Molecular cloning of a gene for a thermostable β-glucosidase from Clostridium thermocellum into Escherichia coli

✍ Scribed by Sunil Kadam; Arnold L. Demain; Jacqueline Millet; Pierre Beguin; Jean-Paul Aubert


Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
486 KB
Volume
10
Category
Article
ISSN
0141-0229

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Molecular cloning of a gene coding for t
✍ Dr. Rainer Borriss; Renate Manteuffel; Jürgen Hofemeister 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 505 KB

The bglM gene DNA coding for a thermostable beta-I .3,1.4-glucanase of Bacillus maceruns E I38 was isolated by direct shot-gun cloning into Escherirhia coli using plasmid pBR322 as a vector. By deletion analysis the bg/M coding region was located within a 1.0 kb region of the cloned Bacillus DNA fra