Characterization and comparison of two newly established epstein-barr virus-negative lymphoma B-cell lines: Surface markers, growth characteristics, cytogenetics, and transplantability

✍ Scribed by Masafumi Abe; Yoshihiro Nozawa; Haruki Wakasa; Hitoshi Ohno; Shiro Fukuhara

• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 822 KB
• Volume: 61
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/1097-0142(19880201)61:3<483::aid-cncr2820610313>3.0.co;2-l

✦ Synopsis

Two Epstein-Barr virus (EBV)-negative lymphoma B-cell lines, HBL-1 and HBL-2, were established from a pleural effusion and a lymph node biopsy of two patients with diffuse large cell lymphoma. HBL-1 and H B L 2 showed the characteristics of activated Bcells in B-cell lineage, as did original lymphoma cells. Chromosome analyses revealed that HBGI exhibiting 14q+ marker-positive lymphoid cancer showed a new subclass of 14q32 translocation resulting from a translocation between chromosomes 14 and 16, which had been masked in a complex translocation involving five chromosomes, and that HBL-2 had a 14q+ marker chromosome, the result of an 11;14 translocation It(l1;14)(q13;32)]. Successful heterotransplantation into athymic nude mice demonstrated tumorigenicity of HBL-1 and HBL-2. The transplantability and tumor growth rate of HBG2 were higher and more rapid than those of HBGI. HBL-1 and HBL-2 appear useful for facilitating therapeutic investigations as well as immunologic and oncogenic studies in B-cell lymphomas.