Characterization and comparison of two newly established epstein-barr virus (EBV)-negative and EBV-positive Burkitt's lymphoma cell lines. EBV-negative cell line with a low level of expression of ICAM-1 molecule and EBV-positive cell line with a high level of expression of ICAM-1 molecule
✍ Scribed by Masafumi Abe; Kazuhiro Tasaki; Kunihiko Tominaga; Shiro Fukuhara; Shosuke Imai; Toyoro Osato; Haruki Wakasa
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 924 KB
- Volume
- 69
- Category
- Article
- ISSN
- 0008-543X
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✦ Synopsis
Two human Burkitt's lymphoma cell lines (HBL-4 and HBL-5) were established individually from two patients with small noncleaved cell lymphoma (Burkitt's type). The HBL-4 cell line is Epstein-Barr virus (EBV)-negative, and the HBL-5 cell line is EBV-positive. Cytogenetically, both cell lines had the same chromosomal translocation, t(8;14)(q24;q32) as those observed in the primary malignant cells from individual patients. Morphologic, immunophenotypic, cytogenetic, and molecular studies confirmed that both cell lines were derived from the primary lymphoma cells in vivo. HBL-4 cells lacked CD23(H107), CDlla(LFA-l), and latent membrane protein (LMP) but expressed CD54(ICAM-1) at low levels, whereas HBL-5 cells showed the high level of expression of CD54 and faint expression of LMP but lacked CDlla. In addition, the EBV-positive lymphoblastoid cell line (LCL) expressed CDlla, CD23, CD.54, and LMP at high levels. Therefore, an HBL-5 phenotype with expression of CD54 and LMP tends toward an LCL phenotype, and the augmentation of CD54 on the HBL-5 cells in comparison