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Characteristics of phospholipase D in reverse micelles of Triton X-100 and phosphatidylcholine in diethyl ether

✍ Scribed by S. Subramani; N. Dittrich; F. Hirche; R. Ulbrich-Hofmann

Publisher
Springer Netherlands
Year
1996
Tongue
English
Weight
297 KB
Volume
18
Category
Article
ISSN
0141-5492

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✦ Synopsis

Phospholipase D, from cabbage, is active in reverse micelles formed from its substrate phosphatidylcholine and Triton X-100 in diethyl ether. The activity is optimum at w0=12.5. The increase of the molar ratio of Triton X-100/substrate from 1:4 to 2:1 resuits in an activity decrease by 25 %. At 136 mM Triton X-100 the KM value in reverse micelles is 136 mM, whereas it is 0.40 mM in the aqueous system containing SDS.

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