Characteristics of Epstein-Barr virus isolated from the malignant lymphomas in Korea

✍ Scribed by Insun Kim; Eui-Ryun Park; Seol-Hee Park; Zhenhua Lin; Young-Sik Kim

• Publisher: John Wiley and Sons
• Year: 2002
• Tongue: English
• Weight: 255 KB
• Volume: 67
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.2193

✦ Synopsis

Abstract

Epstein‐Barr virus (EBV) is a common herpes virus linked to a variety of human neoplasms. In this study, the EBV detection was identified with the paraffin‐embedded tissues from 62 non‐Hodgkin's lymphomas, 20 Hodgkin's lymphomas, and 48 non‐neoplastic tonsils, using PCR for EBNA‐1 and EBER‐1 mRNA in situ hybridization for EBER‐1 mRNA. The isolates were analyzed for type 1/2, variants C/D and F/f, and LMP‐1 30 bp deletion. EBV was isolated in 31 of 48 (66%) non‐neoplastic tonsils, 24 of 42 (57%) B cell lymphomas, in 15 of 20 (75%) T cell lymphomas, and 17 of 20 (85%) Hodgkin's lymphomas. These viruses were classified as type 1 for 81% of non‐neoplastic tonsils, 95% of B cell lymphomas, 93% of T cell lymphomas, and 73% of Hodgkin's diseases. Both type 1 and 2 viruses were isolated in one non‐neoplastic tonsil and 3 Hodgkin's diseases. Type C virus was predominant in non‐neoplastic tonsils (77%) and B cell lymphomas (75%), while type D virus was common in T cell lymphomas (71%) and Hodgkin's diseases (73%) (P < 0.05). Majority of the viruses detected in non‐neoplastic tonsils (93%) and malignant lymphomas (91%) were “F” prototype. LMP‐1 30 bp deletion was found in high frequency in both non‐neoplastic tonsils (92%) and malignant lymphomas (86%). In conclusion, most of EBV found in Korea was type 1, and “DF” genotype was more frequent in T cell lymphomas and Hodgkin's diseases than in non‐neoplastic tonsils and B cell lymphomas. LMP‐1 30 bp deletion did not seem to be associated with malignant lymphomas. J. Med. Virol. 67:59–66, 2002. © 2002 Wiley‐Liss, Inc.