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Characterisation of the gas–liquid mass transfer in shaking bioreactors

✍ Scribed by Ulrike Maier; Jochen Büchs

Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
378 KB
Volume
7
Category
Article
ISSN
1369-703X

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✦ Synopsis

The maximum gas-liquid mass transfer capacity of 250ml shaking flasks on orbital shaking machines has been experimentally investigated using the sulphite oxidation method under variation of the shaking frequency, shaking diameter, filling volume and viscosity of the medium. The distribution of the liquid within the flask has been modelled by the intersection between the rotational hyperboloid of the liquid and the inner wall of the shaking flask. This model allows for the calculation of the specific exchange area (a), the mass transfer coefficient (k(L)) and the maximum oxygen transfer capacity (OTR(max)) for given operating conditions and requires no fitting parameters. The model agrees well with the experimental results. It was furthermore shown that the liquid film on the flask wall contributes significantly to the specific mass transfer area (a) and to the oxygen transfer rate (OTR).

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