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Characterisation of inhibitors of acetylcholinesterase by an automated amperometric flow-injection system

✍ Scribed by Alexander Günther; Ursula Bilitewski

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
702 KB
Volume
300
Category
Article
ISSN
0003-2670

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✦ Synopsis

Acetylcholinesterase was immobilised on magnetic particles and integrated in a flow-injection system via a magnetic reactor. Enzyme activity was determined amperometrically using acetylthiocholine chloride as enzyme substrate. This system was applied to enzyme inhibition tests. Inhibition constants and lower detection limits were determined for carbofuran, paraoxon, malaoxon and paraoxon-methyl. The resulting data were compared to those obtained with a photometric test, i.e. the determination of thiocholine via its reaction with the EIlman's reagent 5,5'-dithio-bis-2-nitrobenzoic acid using the same flow system. As they correlated well to those reported for the native enzyme the flow-injection analysis device can be applied to automated determination and characterisation of enzyme inhibitors.

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