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Changing the Substrate Specificity of Cytochrome c Peroxidase Using Directed Evolution

✍ Scribed by André Iffland; Susanne Gendreizig; Petra Tafelmeyer; Kai Johnsson


Book ID
115588425
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
182 KB
Volume
286
Category
Article
ISSN
0006-291X

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Alteration of the Substrate Specificity
✍ Bettina Lingen; Doris Kolter-Jung; Pascal Dünkelmann; Ralf Feldmann; Joachim Grö 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 136 KB 👁 1 views

## Abstract __Alteration of the substrate specificity of thiamin diphosphate (ThDP)‐dependent benzoylformate decarboxylase (BFD) by error‐prone PCR is described. Two mutant enzymes, L476Q and M365L‐L461S, were identified that accept__ ortho__‐substituted benzaldehyde derivatives as donor substrates