## Abstract __Alteration of the substrate specificity of thiamin diphosphate (ThDP)โdependent benzoylformate decarboxylase (BFD) by errorโprone PCR is described. Two mutant enzymes, L476Q and M365LโL461S, were identified that accept__ ortho__โsubstituted benzaldehyde derivatives as donor substrates
Altering the Substrate Specificity of RhlI by Directed Evolution
โ Scribed by Pavan Kumar Reddy Kambam; Dawn T. Eriksen; Jason Lajoie; Daniel J. Sayut; Lianhong Sun
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 261 KB
- Volume
- 10
- Category
- Article
- ISSN
- 1439-4227
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
## Abstract Of all presently available BaeyerโVilliger monooxygenases, phenylacetone monooxygenase (PAMO) is the only representative for which a structure has been determined. While it is an attractive biocatalyst because of its thermostability, it is only active with a limited number of substrates
We attempted to expand the range of chlorinated solvents degraded by Xanthobacter autotrophicus GJ10 to include trichloroethylene by the rational modification of the enzyme haloalkane dehalogenase. The amino acids Phe164, Asp170, Phe172 and Trp175 were individually replaced with alanine by site-dire