Alteration of the Substrate Specificity
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Bettina Lingen; Doris Kolter-Jung; Pascal Dünkelmann; Ralf Feldmann; Joachim Grö
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Article
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2003
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John Wiley and Sons
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English
⚖ 136 KB
👁 1 views
## Abstract __Alteration of the substrate specificity of thiamin diphosphate (ThDP)‐dependent benzoylformate decarboxylase (BFD) by error‐prone PCR is described. Two mutant enzymes, L476Q and M365L‐L461S, were identified that accept__ ortho__‐substituted benzaldehyde derivatives as donor substrates