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Changes of the p16 gene but not the p53 gene in human chondrosarcoma tissues

✍ Scribed by Julia Asp; Luca Sangiorgi; Sven E. Inerot; Anders Lindahl; Lara Molendini; Maria Serena Benassi; Piero Picci

Publisher: John Wiley and Sons
Year: 2000
Tongue: French
Weight: 70 KB
Volume: 85
Category: Article
ISSN: 0020-7136
DOI: 10.1002/(sici)1097-0215(20000315)85:6<782::aid-ijc7>3.0.co;2-o

No coin nor oath required. For personal study only.

✦ Synopsis

The role of two important tumour suppressor genes, p16 and p53, was evaluated in cartilaginous tumour tissues. Genomic DNA from 22 chondrosarcomas, 5 benign chondroid tumours, 1 sample of reactive proliferative cartilage and 2 samples of normal cartilage were analysed using polymerase chain reaction, single strand conformational polymorphism, DNA sequencing and methylation-specific polymerase chain reaction. The p16 gene was found to be partly methylated in 5 high-grade chondrosarcomas and homozygously deleted in 1 chondrosarcoma. Moreover, a polymorphism was detected in 3 malignant tumours, but not in benign tumours or normal cartilage. Analysis of the p53 gene revealed an unchanged structure in all samples. These findings show a role for p16, but not p53, in chondrosarcoma. Int.

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