𝔖 Bobbio Scriptorium
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Changes of hydration during conformational transitions of DNA

✍ Scribed by M. Harmouchi; G. Albiser; S. Premilat


Book ID
104655092
Publisher
Springer
Year
1990
Tongue
English
Weight
481 KB
Volume
19
Category
Article
ISSN
1432-1017

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✦ Synopsis


Fiber X-ray diffraction and measurement of fiber dimensions yields information about the hydration of DNA in fibers. The results obtained give us the fraction of nucleotides in the B form for the A-B transition or the rate of progression for the B-C transition as functions of the number of water molecules per nucleotide. The present experimental results confirm the importance of cooperativity in the A-B transition and the progressive change of the DNA double helix conformation during the C-B transition. At least twenty additional water molecules per nucleotide are necessary to stabilize the B form for DNA molecules in fibers following the A to B transition whereas only ten are sufficient when the B conformation is obtained starting from the C form.


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