Changes of hydration during conformational transitions of DNA
β Scribed by M. Harmouchi; G. Albiser; S. Premilat
- Book ID
- 104655092
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 481 KB
- Volume
- 19
- Category
- Article
- ISSN
- 1432-1017
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β¦ Synopsis
Fiber X-ray diffraction and measurement of fiber dimensions yields information about the hydration of DNA in fibers. The results obtained give us the fraction of nucleotides in the B form for the A-B transition or the rate of progression for the B-C transition as functions of the number of water molecules per nucleotide. The present experimental results confirm the importance of cooperativity in the A-B transition and the progressive change of the DNA double helix conformation during the C-B transition. At least twenty additional water molecules per nucleotide are necessary to stabilize the B form for DNA molecules in fibers following the A to B transition whereas only ten are sufficient when the B conformation is obtained starting from the C form.
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