Changes in the pattern of histone H1 phosphorylation duringDrosophila hydeiembryogenesis
β Scribed by Nedialka T. Harisanova; Kiril H. Ralchev
- Book ID
- 104768100
- Publisher
- Springer
- Year
- 1986
- Tongue
- English
- Weight
- 317 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0301-4851
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β¦ Synopsis
Histone H1 phosphorylation was examined during embryonic development of Drosophila hydei. A changing pattern of H1 phosphorylation upon separation on an acid-urea polyacrylamide gel was observed in the course of Drosophila embryogenesis. It is considered to be related to the decrease of the mitotic activity of the cells as development proceeds.
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Nucleosomal subunits isolated from rabbit thymus nuclei in 0.04 M K2SO 4-0.02 M Tris, pH 7.4 were devoid of histone H1, while whole chromatin prepared in the same buffer contained the full complement of histone HI. The question is asked why histone H1 dissociates from the subunits but not from the h