## Abstract Denervationβinduced alterations in the composition of the soluble protein population of various stages of forelimb regenerates in the adult newt were examined. Denervation alters the normal protein arrays in three ways: (1) proteins may be eliminated from an array, (2) proteins not norm
Changes in liver metabolites during forelimb regeneration in the adult newt,D. viridescens
β Scribed by Procaccini, Donald J. ;Doyle, Catherine M. ;Procaccini, Robert L.
- Publisher
- John Wiley and Sons
- Year
- 1973
- Tongue
- English
- Weight
- 937 KB
- Volume
- 186
- Category
- Article
- ISSN
- 0022-104X
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β¦ Synopsis
Abstract
Quantitative extraction, histological, and histochemical procedures were used to investigate the effects of the stress of forelimb amputation on liver metabolites in the adult newt, D. viridescens. Results of these experiments showed that hepatic PAS responsive stores were depleted two hours through one week after amputation while protein decreased 24 hours β one week after amputation. There was no change in hepatic lipid. Hepatic GLU 6βPDH and Ξ±βglucan phosphorylase activities increased almost immediately after limb amputation and did not return to normal until one week and two weeks later, respectively. These and previous experiments suggest a systemic response to forelimb amputation whereby liver metabolites may initially provide energy reserves for escape and survival and then contribute to the early phases of regeneration.
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The concentrations of thyroxine (TJ and triiodothyronine (T,) were determined on samples of fresh sera from adult male and female newts during the course of forelimb regeneration by means of radioimmunoassay (RIA) methods. The results show that the circulatory levels of T, and T3 in males and female
## Abstract Cyclic 3β²,5β²βguanosine monophosphate (cGMP) was measured at eight stages of forelimb regeneration in adult newts and compared with the cGMP levels of nonβregenerating control limbs. There was a significant increase in cGMP content during dedifferentiation followed by a sharp decrease to
## Abstract The regenerating and stump tissues of amputated forelimbs of the adult newt, __Diemictylus viridescens__, were analyzed for collagen content and synthesis. Collagen soluble and insoluble in 0.45 M NaCl was assayed by (1) spectrophotometric determination of hydroxyproline, (2) radiotrace
## Abstract Five distinguishable stages of tail regeneration in the adult newt have been established. The stages are based on external morphological changes and internal histological characteristics rather than chronological age of the regenerate.