A quantitative estimation of sodium dodecyl sulphate-extractable plastid envelope polypeptides during greening of Avena sativa L. laminae is described, combined with protein distribution and plastid number studies over the same period. A primary light-dependent and cytoplasm-dependent increase in b
Changes in envelope permeability during chloroplast development
โ Scribed by R. Hampp; H. W. Schmidt
- Publisher
- Springer-Verlag
- Year
- 1976
- Tongue
- English
- Weight
- 392 KB
- Volume
- 129
- Category
- Article
- ISSN
- 0032-0935
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โฆ Synopsis
The permeability of the plastid envelope during the development of Arena saliva plastids was investigated by light scattering and uptake of various labelled compounds (malate, succinate, glutamate, c~ketoglutarate, citrate, glycine, sucrose). The results presented show that a primary event during greening is a change in permeability, thereby allowing an in- creased transport of metabolites across the membranes of very early etio-chloroplast stages. The results are discussed in view of an adaption of the plastid envelope permeability to the changing requirements of externally synthesized precursors and intermediates during development.
๐ SIMILAR VOLUMES
Pulse-chase experiments using [35S]methionine with various light and dark regimes have been used to follow in situ polypeptide accumulation during plastid development in Arena sativa (var. Mostyn). Subsequent isolation and later fractionation of the etioplasts, etio-chloroplasts or chloroplasts into