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Changed properties of the A subunit in DNA gyrase with a B subunit mutation

✍ Scribed by Bogdanova, E. S. ;Mirkin, S. M. ;Shmerling, Zh. G.

Publisher
Springer
Year
1982
Tongue
English
Weight
252 KB
Volume
186
Category
Article
ISSN
0026-8925

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✦ Synopsis

To investigate the interaction of subunits A and B of DNA gyrase during DNA supercoiling, a Cour mutant of Escherichia coli was obtained and the effect of nalidixic acid on the supercoiling of DNA by wild-type and mutant enzymes was assayed. The enzyme of the Cour strain proved to be more sensitive to nalidixic acid than the wild-type DNA gyrase. Hence the mutation affecting the B subunit can also change the properties of the A subunit, which fact suggests that the two subunits of DNA gyrase are in contact during DNA supercoiling.

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