𝔖 Bobbio Scriptorium
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Change in shoot proliferation with repeated in vitro subculture of shoots of woody species of Rosaceae

✍ Scribed by Margaret E. Norton; Colin R. Norton

Book ID
104618453
Publisher
Springer
Year
1986
Tongue
English
Weight
369 KB
Volume
5
Category
Article
ISSN
0167-6857

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✦ Synopsis

Shoots of 6 ornamental species and cultivars of Rosaceae were repeatedly subcultured in vitro for 9 generations on Linsmaier and Skoog (1965) medium with the addition of BA. Shoot proliferation increased over the first few generations and then gradually declined in all species and at all BA concentrations tested with the exception of Chaenomeles/aponiea in which a decline in shoot formation occurred only at 5.0 mg 1-1 BA. A decrease in shoot length and leaf size and an increase in the ineidenc~ of callus formation was observed after several subcultures. This apparently irreversible decline could be due to either genetic or epigenetic change resulting from repeated fluxes in cytokinin, nutrient status or sucrose, or to elimination of seasonal environmental fluctuations.

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