Elution ofTrichoderma reeseicellulase fr
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D. E. Otter; P. A. Munro; G. K. Scott; R. Geddes
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Article
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1984
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Springer Netherlands
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English
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Up to 45% of bound cellulase (Avicelase) could be recovered from crystalline cellulose by pH adjustment with NaOH (0.008-0.01 M) to pH 10.0 (4O'C). Enzyme was simultaneously desorbed and inactivated as the pH increased and both eventsoccurred within 1 minute. Enzyme desorption and stability were als