✦ LIBER ✦
Elution ofTrichoderma reeseicellulase from cellulose by pH adjustment with sodium hydroxide
✍ Scribed by D. E. Otter; P. A. Munro; G. K. Scott; R. Geddes
- Book ID
- 104637301
- Publisher
- Springer Netherlands
- Year
- 1984
- Tongue
- English
- Weight
- 322 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0141-5492
No coin nor oath required. For personal study only.
✦ Synopsis
Up to 45% of bound cellulase (Avicelase) could be recovered from crystalline cellulose by pH adjustment with NaOH (0.008-0.01 M) to pH 10.0 (4O'C). Enzyme was simultaneously desorbed and inactivated as the pH increased and both eventsoccurred within 1 minute. Enzyme desorption and stability were also temperature dependent and desorption increased to approximately 65% by adding detergents.