Cellulose acetate membrane electrophoresis in the analysis of urinary proteins in patients with tubulointerstitial nephritis

✍ Scribed by Ryo Kubota; Ryoko Machii; Nobuo Hiratsuka; Osamu Hotta; Yoshihisa Itoh; Shizuko Kobayashi; Kiyoko Shiba

• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: English
• Weight: 256 KB
• Volume: 17
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.10066

✦ Synopsis

Abstract

Urinary proteins from 14 patients with tubulointerstitial nephritis were analyzed by cellulose acetate membrane electrophoresis. Urinary total protein concentrations were measured, and urinary 15 proteins (prealbumin, albumin, α~1~‐microglobulin, α~1~‐antitrypsin, α~2~‐macroglobulin, haptoglobin, retinol binding protein, transferrin, β~2~‐microglobulin, IgA, IgG, κ‐ and λ‐light chains, cystatin C, and lysozyme) were identified by the use of a rapid and highly sensitive colloidal silver staining reagent suited for use with cellulose acetate membranes, as reported previously by Matsuda et al. (J Clin Lab Anal 15:171–174, 2001; __Clin Chem__47:763–766, 2001) and Hiratsuka et al. (J Clin Lab Anal 10:403–406, 1996). We also analyzed urinary total protein concentration and urinary protein fractions according to the presence of acute or nonacute interstitial nephritis. In addition, the relationship between urinary protein fraction and complications of interstitial nephritis was analyzed. The goal of this work was to find a useful index for the diagnosis of tubulointerstitial nephritis. J. Clin. Lab. Anal. 17:44–51, 2003. © 2003 Wiley‐Liss, Inc.