Cellulolytic enzymes for the hydrolysis of leached beet cosette

✍ Scribed by Ines Contreras; Renata Gonzalez; Anamaria Ronco; Juan A. Asenjo

• Publisher: Springer Netherlands
• Year: 1982
• Tongue: English
• Weight: 389 KB
• Volume: 4
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00139282

✦ Synopsis

SUM~ARY

Cellulolytic fungi

were isolated from rotting leaves and tested for extracellular cellulase activities (CMCase, avicelase, cel]obiase and xylanase). The effect of the proportion of the enzyme activities on the rate of degradation of leached beet cosette was observed using a range of supernatant fluids in appropriate combinations.

At low cosette concentrations (1.5-3.0 g/l), avicelase and cellobiase were the rate limiting enzymes; avicelase in the initial stages of reaction and cellobiase after 6-8 hours, when cellobiose inhibition becomes important.

A ratio of celiobiase to avicelase of approx 2.0 was established as appropriate.

At higher substrate concentrations (I0 g/l, 40 g/l) the best cellobiase to avicelase ratio was maintained and up to 40% hydrolysis was obtained in the i0 g/l incubation with I0 uav/l and 20 Ucellob/l . At I00 g/l cosette concentration, substrate inhibition was observed.